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    Cytokine deviation is one of the mechanisms of allogenic kidney transplantation immune tolerance induced by hydrocamptothecin

    SHEN Ruiª²Xiong, SHAO Guoª²Xing, WANG He, SHI Bingª²Yi,WANG Qiang

    Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi¬ðan 710033, China, Department of Urology, 309 Hospital of Chinese PLA, Beijing 100091, China£¬ Department of Urology, 465 Hospital, Jilin Military Medical College£¬Fourth Military Medical University, Jilin 132000, China

    ¡¾Abstract¡¿AIM£º To induce immune tolerance in recipient rats of allogenic kidney transplantation with hydrocamptothecin (HCPT) and cyclosporin A (CsA) and to study the mechanism of the tolerance. METHODS: The inbred DA rats were chosen as kidney donors and Lewis rats as recipients. After transplantation, the recipients were treated with HCPT and/or CsA. The levels of cytokine expression were assessed using RTª²PCR. RESULTS: 4 out of 10 in group C (HCPT 2.0 mg) and 5 out of 10 in group E (HCPT+CsA) survived longer than 150 days even immunosuppression (HCPT and CsA) was withdrawn at 30 days after transplantation. The levels of IL2 and IFN¦ÃmRNA expression were significantly lower in the tolerant rats than those in rejected rats. The levels of IL4 and IL10 mRNA were significantly higher in the tolerant rats than those in rejected rats. The level of cytokine mRNA expression in the grafts was similar to that in the spleen. CONCLUSION: High dosage of HCPT or low dosage of HCPT combined with CsA can induce tolerance in allogenic kidney transplantation in the recipients. Cytokine deviation is in some way responsible for the allogenic kidney transplantation immune tolerance induced by hydrocamptothecin. The level of cytokine mRNA expression in the grafts is similar to that in the spleen of recipients. ......