Establishment and biological characteristics of a human bladder cancer cell line BCª²6

    LU Jiang, SHI Changª²Hong, CHEN Jiang, CHAI Qingª²Huan, LIU Fang, WANG Boª²Liang, CAO Yiª²Zhan, TIAN Xiaoª²Xi, HE Baoª²Jian

    1Department of Emergency Medicine, 4Department of Nephrology, Tangdu Hospital, Fourth Military Medical University, Xi¬ðan 710038, China, 2Department of Microbiology, School of Basic Medicine, Fourth Military Medical University, Xi¬ðan 710033, China, 3Department of Emergency Medicine, Shaanxi Aerospace Hospital, 5Department of Clinic, Storehouse of Medicinal Materials, Jinan Military Command

    ¡¾Abstract¡¿ AIM: To establish a new bladder cancer cell line and to provide a useful model for further research of bladder cancer. METHODS: Of the 26 specimens of human bladder cancer, 18 specimens were obtained by asepsis surgery and then cut into fragments and cultured, 8 specimens were homogenated to cell suspension and cultured and 12 specimens were implanted subcutanousely (SC) into nude mice. The transplanted tumor specimens grew SC and were passaged in nude mice for three generations and then the tumor tissues were cultured in vitro. RESULTS: Tissue culture and cell suspension culture failed to establish the bladder cancer cell line. Of the 12 subcutanousely implanted tumor specimens, three were found growing SC in three nude mice, respectively, but one of which died incidentally and the tumor disappeared in another after the second passage. Only one tumor grew SC at relatively more rapid speed, part of which, after three passages in the nude mice, was taken out and cultured in vitro, thus a bladder cancer cell line BCª²6 was established. The established cell line resembled the original in tumor morphology and differentiation degree. The mode range of cell chromosome was 66-72. The cell cycle analysis showed that G1 was 0.58, G2 0.08 and S1 0.35. The cell population doubling time was 44 h. CONCLUSION: A stable cell line has been established, for the bladder cancer cell line BCª²6 is similar to the primary tumor in the biological characteristics and has been cultured in vitro continually for more than one year without characteristic changes. ......