Induction of specific CTLs in BALB/c mice by chiral MUC1 synthetic peptide vaccine

    WANG Yi, SU Xiaoª²Yun, WANG Fang, YU Hong, CHANG Shuª²Fang, YAN Weiª²Qun

    1Department of Biochemistry, Institute of Frontier Medical Science, Jilin University, Changchun 130021 China, 2Blood Bank, Cancer Hospital of Jilin Province, Changchun 130012, China

    ¡¾Abstract¡¿ AIM: To study the induction of MUC1ª²specific CTL in mice by peptide vaccine that was composed of mouse serum albumin and synthetic peptide Dª²MUC1 with partial chirality. METHODS: The Dª²MUC1 peptide containing Dª²Ser was synthesized with solid phase peptide synthesis, purified and coupled with mouse serum albumin. BALB/c mice were vaccinated by Dª²MUC1 peptide vaccine and GMª²CSF or thymopeptide (TP). The abilities to stimulate the proliferation of lymphocytes and to induce the peptideª²specific CTL were analyzed by 3Hª²TdR incorporation and release assay, respectively. RESULTS: The molecular weight of the target peptide was confirmed by mass spectrum (MS) analysis. The Dª²MUC1 peptide vaccine stimulated the proliferation of lymphocytes significantly. MUC1ª²specific CTLs could be induced by Dª²MUC1 peptide vaccine and these CTLs could kill the MUC1ª²positive GZ.A5.3H.4 cells. CONCLUSION: The chiral Dª²MUC1 peptide vaccine can induce MUC1ª²specific CTL in mice, which provides some evidence for the treatment of tumors with synthetic Dª²MUC1 peptide vaccine with partial chirality. ......