2003, Vol. 24, No. 11 食品科学 ※营养卫生 126

    高空环境条件对鸡冠花抗氧功能的影响

    翁德宝1 ， 汪海峰2

    (1. 江苏教育学院生物化学教研室，南京 210013 ；2. 南京财经大学粮油食品质量检测中心，南京

    210003)

    摘 要 ： 探讨高空环境条件对鸡冠花抗氧化功能的影响。 方法 ： 利用高空气球搭载了鸡冠花团穗黄品种( Celosia

    cristata L.cv .Spicata Tuansuihuang) 的种子， 进行空间诱变处理。 飞行高度40.112Km ， 飞行时间近4h ， 回收后

    播种栽培，采收子一代(SP1) 花序，茎叶。在·OH 、O 2-·自由基的产生和检测系统中，分别加入一定量的

    搭载组和对照组花序、 茎叶四种水提取液， 通过比色法观察其与活性氧引发反应的竞争效应。 同时， 分别测

    定分析了各组样品的VC 、 黄酮醇的含量和SOD 活力， 据此探索在高空环境影响后样品抗氧化活性变化与自由

    基清除剂含量之间的关系。结果：各组水提取液均能降低·OH 引发的还原型细胞色素C[Cyt ·C( Ⅱ)] 氧化作

    用和O 2 -·对羟胺的氧化作用， 而两种搭载组样品水提取液清除活性氧的能力均比对照组显著提高， 并与其自

    由基清除剂含量和活力的增高相关。 结论：高空环境诱变处理对鸡冠花中抗氧化活性物质的合成和抗氧化功能

    产生了显著效应。

    关键词：鸡冠花；高空环境；活性氧；清除作用；诱变效应

    Effect of High Altitude Environment on the Antioxidation of

    Water Extract from Celosia cristata L.

    WENG De-bao 1 ，WANG Hai-feng 2

    (1.Department of Biochemistry Jiangsu Education College, Nanjing 210013, China ； 2.Anal Test Center of Oil

    Foodstuffs,Nanjing University, Nanjing 210003, China)

    Abstract ： Objective: To investigate the effect of high altitude environment condition on the antioxidation of water extracts from

    Celosia cristata L.Methods: The seeds of C. cristata L. cv. Spicata Tuansuihuang were carried by a high-altitude balloon (CHAB)

    for space mutation, flying to an altitude of 40.112 km from sea level for about 4 hours. After returning to the ground, the treated

    seeds were cultivated on the ground and the inflorescences and leaves of SP1 generation were collected . Adding 4 kinds of the

    water extracts of the inflorescences and leaves of CHAB groups and the control groups respectively to the hydroxyl radical

    ( · OH) and superoxide radical (O 2 - · ) generating and detecting systems for the observation on their competitive abilities to active

    oxygen reactions teste by colorimetric method . The contents of VC, flavonols and activity of SOD of all samples were also

    analyzed and determined respectively . Then, the relationship between the antioxidation activity of samples and the contents

    and activity of free radical scavengers in samples was investigated for the effect of high altitude environment . Result: The water

    extracts of all samples could reduce · OH initiated oxidation of reductive Cyt · c( Ⅱ) and inhibit O 2 - · initiated oxidation of

    hydroxylamine. But, the copabilities of scavenging active oxygen of 2 kinds of water extracts from CHAB groups were

    significantly higher than those of the control. The obvious positive correlationship existed between the above results and also

    between the increase of the contents and the activity of free radical scavengers in the samples of CHAB . Conclusion: The

    evident effects of high space environment condition mutation on the synthesis of the antioxidation active substances and the

    increase of antioxidation capability in C. cristata L. were observed.

    收稿日期：2003-03-31

    基金项目 ： 江苏省资源生物技术重点实验室开放研究基金资助(200013)

    作者简介：翁德宝(1947-) ，男，教授，主要研究方向为营养生物化学及植物资源的开发利用。127 ※营养卫生 食品科学 2003, Vol. 24, No. 11

    随着航天空间科学技术的飞跃发展，人类开拓了空

    间植物学的新领域，研究空间环境特殊的条件对植物的

    影响。许多研究成果证明，宇宙空间的高能重离子辐

    射、微重力、超真空、超洁净等物理条件深刻影响植物

    的生长、发育、衰老及遗传变异。自20 世纪 80 年代，我国科技人员又采用高空气球搭载了小麦、水稻、谷

    子、青椒、大豆等农作物种子，开展高空环境诱变育种

    的研究实验，同样也取得了显著的成果。在近地高空环

    境，存在很强的宇宙线高能核粒子辐射、强烈的紫外线

    照射和极稀薄的空气等特殊条件 ......