而iNOS催化L2精氧酸与 O2 作用生成一氧化氮(NO)

    增加。促进 NO 产生是虫草在 VMC发挥保护作用的

    机制之一: (1)现已证明 NO 抑制病毒蛋白酶 3C和病

    毒蛋白酶 2A ,抑制病毒复制 ,并通过降低细胞代谢和

    抑制凋亡保护宿主细胞[10 ]; (2) NO 舒张血管平滑肌 ,使炎症区血管扩张 ,增加血供 ,促进炎症消散吸收 ,减

    轻心肌损害。

    参考文献

    1 Wessely R , Henke A , Zell R , et al . Low2level expresion of a mutant

    coxsackieviral cDNA induces a myocytopathic effects in culture cells :an ap2

    proach to the study of enteroviral persistence in cardiac myocytes. Circula2

    tion ,1998 ;98∶ 450～457

    2 Beck MA. Nutitionally induced oxidative st ress : effects on viral disease.

    Am J Clin Nut r ,2000 ;71 (suppl)∶ 1676s～1679s

    3 Hiroka Y, Kishimoto G, Takada H ,et al . Role of oxygen derived f ree

    radicals in the pathogenesis of coxackievirus B3 myocarditis in mice. Cardio2

    vasc Res ,1993 ;27∶ 957～961

    4 邱德凯,靖大道,萧树东,等. 冬虫夏草多糖脂质体对肝炎后肝硬化

    患者 T细胞免疫调节作用研究.中华消化杂志,1995 ;15∶ 265～267

    5 徐仁和,彭祥鄂.冬虫夏草对天然杀伤细胞活性及肺瘤克隆形成的

    影响. 湖南医学院学报,1988 ;13∶ 107～111

    6 王龙妹,傅惠娣,周志兰. 冬虫夏草对小鼠巨细胞病毒感染的作用.

    中国临床药学杂志,1999 ;8∶ 109～111

    7 王向宇,吴可贵,林经安. 病毒性心肌炎小鼠模型的建立. 福建医学

    院学报,1995 ;29∶ 295～297

    8 龚蕴贞,胡涛,黄升海,等.柯萨奇B3m 病毒感染Balb c 鼠接种浓度

    的研究. 安徽医科大学学报,1997 ;32 (1)∶ 4～7

    9 Shioi T ,Matsumori A ,Nishio R ,et al . Protective role of interleukin212

    in viral myocarditis. J Mol Cell Cardio ,1997 ;29∶ 2327～2334

    10 Zaragoza C , Ocampo CJ ,Saura M ,et al . Inducible nit ric oxide synthase

    protection against coxsackievirus pancreatitis. J Immunol ,1999 ;163∶ 5097

    ～5504

    Effect of Cordyceps sinensis alcohol extraction on acute viral myocarditis

    Zhu Zhaojing , Li Feng1

    , Rao Bangfu1

    (Department of Pharmacy , Chongqing Medical University , Chongqing 400016 ;

    1

    No 1 At tached Hos pital of Chongqing Medical University)

    Objective : To investigated the effect of cordyceps sinensis alcohol ext raction (CASE) on acute viral myocarditis (VMC) . Methods : Acute

    VMC model was made in mice with Coxsackievirus B3(CVB3) int raperitoneal inoculation. iNOS in heart was examined immuno 2chemically.

    Histological changes were determined microscopically. Results : Myocardial necrosis semi quantity score were significantly lower (P < 0. 01)

    for CASE intervention group , and iNOS density in heart were significantly higher (P < 0. 01) . Conclusions : CSAE has significant protection

    on acute VMC , and its possible mechanisms include induction of ex pression of iNOS in heart and increase of NO production.

    Key words viral myocarditis ; cordyceps sinensis ; iNOS

    正交设计法对川芎镇痛活血有效部位的分析X

    杨 薇 曾 瑾 王晓阳 张爱军

    (四川省中药研究所 成都 610041)

    提 要 用正交设计法对川芎四个提取部位 Ⅰ、Ⅱ、Ⅲ、Ⅳ进行了凝血时间、 ADP诱发血栓形成及扭体法、镇痛筛选试验 ,初步

    确定其活血、镇痛的有效成分在 Ⅰ号提取部位。

    关键词 正交设计法;川芎;活血;镇痛

    川芎含挥发油、生物碱、酚性物质、中性物质(内酯

    类) 、有机酸等。本文对川芎四个不同的提取部位进行

    了活血、镇痛方面的正交设计试验 ,以期确定其镇痛、活血的有效提取部位。

    1 材料

    1. 1 试验药物 川芎提取部位 Ⅰ(石油醚) ,100g 原

    生药相当于 5128g样品;川芎提取部位 Ⅱ(乙酸乙酯) ,100g原生药相当于 2103g 样品;川芎提取部位 Ⅲ(甲

    醇) ,100g 原生药相当于 3183g 样品;川芎提取部位 Ⅳ

    (水) ,100g 原生药相当于 27132g 样品。以上样品均

    由本所药化室提供 ,批号 20021225。阿斯匹林原料

    药 ,成都制药厂出品 ,批号 9901023。川芎素(阿魏酸

    42 中药药理与临床 2005 ;21 (1)

    X 四川省科技厅资助项目

    ? 1995-2005 Tsinghua Tongfang Optical Disc Co., Ltd. All rights reserved.钠)片 ,50mg片 ,成都制药一厂出品 ,批号 20020701 ......