应用基因表达谱芯片筛选胃腺癌相关基因
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李 曼, 赵作伟, 辛 彦
胃腺癌;基因表达谱芯片;差异表达基因,李曼,辛彦,赵作伟,李曼,通讯作者:,Screeningofgenesdifferentiallyexpressedi
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李曼, 辛彦, 中国医科大学附属第一医院肿瘤研究所 辽宁省沈阳市 110001
赵作伟, 大连医科大学附属第一医院普外科 辽宁省大连市 116023
李曼, 女, 1973-02-19生, 辽宁省本溪人, 汉族, 2004年中国医科大学博士生, 就职于大连医科大学附属第二医院肿瘤科, 主要从事胃癌转移的研究.
通讯作者: 辛彦, 110001, 辽宁省沈阳市和平区南京北街155号, 中国医科大学附属第一医院肿瘤研究所. yanxin90@yahoo.com
电话：024-23256666-6351
收稿日期: 2005-12-14 接受日期: 2006-01-25
Screening of genes differentially expressed in gastric adenocarcinoma by gene expression profiling technique
Man Li, Zuo-wei Zhao, Yan Xin
Man Li, Yan Xin, Cancer Institute, the First affiliated Hospital of China Medical University, ShenYang 110001, Liaoning Province, China
Zuo-wei Zhao, Department of General Surgery, the First Affiliated Hospital of Dalian Medical University, Shenyang110011, Liaoning Province, China
Correspondence to: Dr. Yan Xin, Cancer Institute, the First Affiliated Hospital of China Medical University, 155 Nanjing North Street, Shenyang 110001, Liaoning Province, China. yanxin90@yahoo.com
Received: 2005-12-14 Accepted: 2006-01-25
Abstract
AIM: To investigate the genes differently expressed between gastric adenocarcinoma and normal mucosal tissues by cDNA microarray technique.
METHODS: The total RNA was extracted from gastric adenocarcinoma and normal mucosal tissues and then purified. The cDNA was obtained by reverse transcription polymerase chain reaction (RT-PCR), and then labeled with Cy5 and Cy3 fluorescence as probes, which were hybridized with gene chip containing 14 784 human 14KcDNA. Subsequently, the signal images were scanned by Agilent fluorescence scanner and analyzed by Pix QuantArray software.
RESULTS: Of the 14 784 target genes, 29 genes were screened out for differences in gene expression level between gastric adenocarcinoma and normal gastric mucosa. Ten of the 29 genes were up-regulated, of which 2 were with unknown function, and 19 were down-regulated ......
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