关键词: 酪氨酸酶相关蛋白-2;克隆;融合表达;白癜风

    摘 要:目的 克隆酪氨酸酶相关蛋白-2(TRP-2)编码基因，表达TRP-2蛋白. 方法 从培养的人黑素细胞中提取总RNA，反转录成cDNA，通过PCR方法扩增TRP-2编码基因，克隆至pUC19载体并测序，再亚克隆至谷胱甘肽巯基转移酶(GST)融合表达载体pGEX-4T-1，转化大肠杆菌，IPTG诱导表达TRP-2/GST融合蛋白. 结果 从培养的人黑素细胞中扩增出编码TRP-2的cDNA，序列测定证实与文献报道的序列一致;成功构建了GST融合表达载体pGEX-4T-1/TRP-2;表达了TRP-2/GST融合蛋白. 结论 成功克隆了人TRP-2基因，构建了GST融合表达载体并表达了TRP-2/GST融合蛋白.

    Cloning and expression of human tyrosinase related protein┐2cDNA

    WANG Qiu-Feng GAO Tian-Wen LI Chun-Ying SHEN Zhu LI Qiang

    1 Center of Dermatology&Venereology of Chinese PLA，Xijing Hospital，Xi'an710033，China，

    2 Department of Dermatology，Tangdu Hospital，Fourth Military Medical University，Xi'an710038，China

    Keywords:tyrosinase related protein-2;clone;fusion ex-pression;vitiligo

    Abstract:AIM To clone the cDNA of human tyrosinase re-lated protein2(TRP-2)and express the corresponding pro┐tein.METHODS Total RNA was isolated from cultured hu-man pigmental cells and mRNA was reversly transcribed into cDNA.Then PCR was used to amplify the TRP-2coding re-gion.The PCR product was cloned into pUC19plasmid and sequenced ......