Estrogen receptor polymorphism in Han population of Shaanxi Province

    LI Junª²Lin, SHU Qing, ZHENG Qiangª²Sun£¬FENG Lei

    1Department of Medical Genetics and Development Biology£¬School of Basic Medcine, Fourth Military Medical University, Xi¡¯an 710033, China£¬ 2Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi¡¯an 710038, China

    ¡¾Abstract ¡¿ AIM: To study the distribution of ER intron 1 polymorphism in Han population of different ages and sexes in Shaanxi Province. METHODS: Amplificationª²Restriction Fragment length Polymorphism (Ampª²RFLP) was used to analyze the polymorphism of Han population in Shaanxi Province. The templates of PCR were extracted from 162 independent cases of different ages and sexes in Shaanxi Province. RESULTS: ¢Ù The full length of ER intron I after PCR was 1.3 kb. ¢Ú Using restriction endonuclease enzymes, three types of genotypes were obtained: Pvu II: P/P (1.3 kb), P/p (1.3 kb, 850 and 450 bp) and p/p (850 bp, 450 bp). ¢Û Using restriction endonuclease enzymes, another three types of genotypes were obtained: Xba I : X/X (1.3 kb), X/x (1.3 kb, 910 and 390 bp)and x/x(910 bp, 390 bp). CONCLUSION: It is suggested that ER gene intron 1 of Han population in Shaanxi Province is polymorphism. The distribution of the genotype is not related to different age groups and different gender groups. ......