关键词: Bcl-2;砷化物;凋亡;雄黄;白血病，髓样，慢性

    摘 要:目的 研究Bcl-2和Bax在硫化砷诱导的K562细胞凋亡中的表达，探讨其在硫化砷诱导的细胞凋亡中的作用. 方法 K562细胞经一定剂量硫化砷作用特定时间后，MTT法检测细胞生长、增殖状态，光镜及透射电镜下观察细胞形态及凋亡情况，S-P免疫组化染色检测Bcl-2、Bax的表达并进行图像分析. 结果 硫化砷对K562细胞生长增殖有时间剂量依赖性抑制作用;175～1400μg·L-1 硫化砷作用18～144h后，K562细胞出现凋亡细胞的形态学改变;免疫组化染色表明K562细胞低表达Bcl-2蛋白(A值为0.152±0.023，阴性对照A值0.108±0.012)，硫化砷处理后对其表达无明显影响(A值为0.138±0.028，P>0.05).硫化砷作用后，Bax蛋白的表达量A值由0.240±0.013增加到0.316±0.021(P<0.01). 结论 硫化砷可抑制K562细胞的生长和增殖并诱导其发生凋亡，其机制可能与Bax蛋白上调有关.

    Expression of Bcl┐2and Bax in arsenic sulfide in┐duced┐apoptosis of K562cell

    CHEN Si-Yu，LIU Shan-Xi，LI Xin-Min

    First Hospital，Xi'an Jiaotong Uiversity，Xi'an710061，China

    Keywords:proto-oncogene proteins c-Bcl-2;arsenicals;apoptosis;realgar;leukemia，myeloid，chronic

    Abstract:AIM To study the expression and the effects of Bcl-2and Bax protein in apoptosis of K562cell induced by ar-senic sulfide.METHODS After being incubated in culture medium containing variable levels of arsenic sulfide，cell growth and proliferation of K562cell was analyzed with MTT assay;apoptosis was detected with cell morphology by light and transmission electron microscopy.The expression of Bcl-2and Bax protein was determined with S-P immuno-histochemical stainning and imaging analysis.RESULTS After the treatment ......