关键词: 帕金森病;神经节苷脂类;脱噬作用

    摘 要:目的 探讨神经节苷脂(ganglioside GM1)对帕金森病(Parkinson's disease，PD)的治疗机制. 方法 以左旋多巴(L-dopa)处理的PC12(大鼠肾上腺嗜铬细胞瘤)细胞为多巴胺(Dopamine，DA)神经元的细胞凋亡模型，应用透射电镜、DNA琼脂糖凝胶电泳、流式细胞仪及免疫组化等技术，观察GM1对凋亡中的PC12细胞的影响. 结果 24h后空白对照组凋亡率为2.5%，200μmol L-1 L-dopa处理组的凋亡率为61.6%;100，50和10μmol L-1 的GM1各加200μmol L-1 L-dopa处理组24h的凋亡率分别为29.7%，52.3%，55.7%，与L-dopa处理组比较有显著差异(P<0.05). 结论 在一定浓度范围内的GM1可抗L-dopa诱导的PC12细胞的凋亡，并提示GM1抗凋亡作用可能在于其对神经细胞膜的保护.

    nti┐apoptotic effect on PC12cell induced by GM1ganglioside

    WU Xiao-Jun GAO Guo-Dong LU Bao-Zhen LI Li-Hong

    1 Department of Neurosurgery，Tangdu Hospital，Fourth Military Medical University，Xi'an710038，China，

    2 Department of Histology and Embryology，Faculty of Preclinical Medicine，Fourth Military Medical University

    Keywords:parkinson's disease;gangliosides;apoptosis

    Abstract:AIM To explore the mechanism of the protection effect of the ganglioside GM1in the treatment of Parkinson's disease.METHODS We built a cellular apoptotic model with PC12cells treated with L-dopa，using the flow cytome-try，electron microscope and electrophoresis immuno-histo-chemical technology to observe whethe the GM1could pro-tect the PC12cells from apoptosis.RESULTS The ratios of apoptosis of the control group and GM1-treated group at the ranges of100 ......