关键词: 螺杆菌，幽门;cagA基因;胃肿瘤

    摘 要:目的 了解胃癌组织HpcagA基因感染状况，探讨HpcagA基因与胃癌的关系. 方法 采用Warthin-Starry银染和原位PCR方法，检测慢性浅表性胃炎(CSG)35例、慢性萎缩性胃炎(CAG)40例和胃癌(CG)35例的胃粘膜Hp及cagA基因感染状况.观察非癌患者胃粘膜炎症反应程度. 结果 慢性浅表性胃炎、萎缩性胃炎、癌组织、癌旁胃粘膜组织Hp检出率分别为57%，62%，0%和54%;cagA基因检出率分别为20%，50%，29%和46%，萎缩性胃炎、癌旁组cagA基因检出率显著高于浅表性胃炎组(P<0.01).非癌患者胃粘膜单个核细胞、分叶核细胞浸润程度cagA+ 组较cagA- 组、Hp+ 组较Hp- 组显著增多(P<0.01)，且cagA+ 组还较Hp+ 组明显增多(P<0.05). 结论 萎缩性胃炎、胃癌患者cagA菌株感染率显著高于慢性浅表性胃炎组.cagA菌株引起更严重的炎症反应.说明HpcagA菌株感染与萎缩性胃炎、胃癌关糸密切.

    Expression of HpcagA gene in gastric carcinoma tis┐sues

    ZHANG Yong，ZENG Wei-Zheng，JIANG Ming-De，CHEN Xiao-Bin，XU Hui，CHEN Hong-Bin Department of Gastroenterology，General Hospital of Chengdu Command Area of Chinese PLA，Chengdu610083，China

    Keywords:Helicobacter pylori;cagA gene;stomach neo-plasms

    Abstract:AIM To examine Helicobacter pylori(Hp)cagA gene infection in gastric cancer tissues so as to study the rela-tionship between Hp cagA gene and gastric cancer.METHODS Warthin-Starry silver staining was used to de-tect Hp，in situ polymerase chain reaction(PCR)to detect

    Hp cagA gene infection in gastric mucosa from35cases of chronic superficial gastritis ......