关键词: 心力衰竭，充血性;肾素-血管紧张素系统;膳食，限钠

    摘 要:目的 观察充血性心衰大鼠严格限钠后循环与局部肾素-血管紧张素系统(RAS)的变化. 方法 将充血性心衰大鼠随机分为3组:心衰组、心衰限钠组、心衰补钠组，假手术大鼠为对照组，用放射免疫分析法和原位杂交技术分别测定各组血浆和心肌血管紧张素Ⅱ(AngⅡ)，醛固酮(Ald)含量及心肌血管紧张素原mRNA表达水平. 结果 心衰限钠组血浆AngⅡ，Ald含量[(225±20)ng·L-1 和(476±62)μg·L-1 ]及心肌AngⅡ含量[心房(20.1±4.5)ng·g-1 ，心室(27.3±5.9)ng·g-1 ]显著高于心衰组[(180±29)ng·L

    -1 ，(248±58)μg·L-1 及(17.5±3.6)ng·g-1 ，(20.1±3.7)ng·g-1 ](P<0.05，P<0.01)，心室肌Ang原mRNA表达水平(12.6±2.3)也显著高于心衰组(8.6±1.7)(P<0.05)，血钠较心衰组下降5%(P<0.05);心衰补钠组心肌血管紧张素原mRNA表达水平与心衰组无显著差别，其余指标与对照组无显著差别. 结论 心衰后限钠进一步激活循环和局部RAS，加重钠水潴留.

    Changes of renin┐angiotensin system of rats with congestive heart failure after restriction of sodium intake

    ZHANG Su-Zhen XU Yong WAN Yu YANG Gang

    1 Department of Pathophysiology，

    2 Department of Physiology，Guangdong Medical College，Guang-dong Zhanjiang524023，China，

    3 Department of Physiology，Medical College of Wuhan University，Hubei Wuhan430071，China

    Keywords:heart failure，congestive;renin-angiotensin sys-tem;diet，sodium restricted

    Abstract:AIM To observe Changes of circular and local renin-angiotensin system(RAS)of rats with restriction of sodium intake after congestive heart failure.METHODS Congestive heart failure(CHF)rats were divided into three groups with sham operation rats group as control.Radioim-munassay and in situ hybrdization techniques were used to de-termine the plasma and myocardium angiotensionⅡ(AngⅡ)and aldosterone(Ald)contents and the expression of an-giotensionogen mRNA ......