关键词: 肝肿瘤;肝炎抗原;C2基因

    摘 要:目的 研究蛋白翻译起始因子C2在肝硬化(LC)、原发性肝细胞癌(HCC)中表达的意义，并探讨其与HBxAg表达的关系. 方法 用免疫组化方法检测C2，HBxAg在肝硬化(n=27)，HCC(n=60)中蛋白水平的表达.Western blot法检测C2蛋白在HCC及其癌旁组织中的表达. 结果 在27例LC中，C2蛋白阳性率为77.8%(21/27)，HBxAg阳性率为74.1%(20/27).60例HCC及42例癌旁组织中，C2蛋白阳性率分别占27.3%(17/60)和83.3%(35/42).在HBxAg阳性的49例HCC中，10例C2蛋白阳性，阳性率为20.4%，在HBx-Ag阴性11例HCC中，7例C2蛋白阳性，阳性率63.6%.C2蛋白在LC中的表达明显高于HCC(P<0.01)，C2蛋白在HCC癌旁组织中表达明显高于癌组织(P<0.01);C2在HBxAg阳性HCC中表达明显低于HBxAg阴性HCC(P<0.05).Western blot与免疫组化结果一致. 结论 C2蛋白表达下调及HBxAg对C2的抑制作用可能与HCC发生发展有关.

    Relationship between C2gene and HBxAg expres┐sion in hepatocellular carcinoma tissue

    ZHANG Qing LIU Jie LI Qing LI Qin WANG Xin QIAO Tai-Dong FAN Dai-Ming

    1 Institute of Digestive Diseases of Chinese PLA，Xijing Hospital，

    2 Department of Pathology，Faculty of Preclinical Medicine，Fourth Military Medical University，Xi'an710033，China，

    3 Department of Radiotherapy，Shaanxi Provincial Cancer Hospital，Xi'an710061，China

    Keywords:liver neoplasms;hepatitis antigens;C2gene

    Abstract:AIM To study the expression significance of pro-tein translation initiation factor C2and its correlationship with expression of HBxAg in liver cirrhosis(LC)and hepato-cellular carcinoma tissues(HCC).METHODS ABC im-munohistochemical method was used to detect the expression of C2protein and HBxAg in27samples LC ......