关键词: 关节/细胞学软骨细胞;细胞培养;胰岛素样生长因子-1;细胞周期

    摘 要:目的 了解胰岛素样生长因子-1(IGF1 )对体外生长的兔关节软骨细胞的生物学效应，为组织工程构件软骨提供理论基础. 方法 取第3代兔关节软骨细胞体外单层培养，培养液DMEM中以终浓度分别为1，10，100μg L-1 的IGF1 各作用细胞2，4及6d，以MTT法检测细胞的增殖情况，并在100μg L-1 IGF1 作用下，采用流式细胞技术进行细胞周期亚时相分析.同时，检测基质中糖醛酸的变化反映蛋白多糖含量的变化. 结果 结果显示在1～100μg L-1 浓度下IGF1 能明显促进培养软骨细胞的增殖，且以100μg L-1 作用4d刺激效果最明显;并能显著影响细胞外基质中糖醛酸的含量. 结论 IGF1 对培养软骨细胞以剂量时间依赖性方式刺激其增殖及功能代谢.

    IGF1 has to do with cultured rabbit articular chon-drocyes

    LIU Gang，HU Yun-Yu，HAN Yi-Sheng

    Institute of Orthopaedics of Chinese PLA，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:joints/cytology chondrocytes;cell culture;in-sulin-like growth factorⅠ;cell cycle

    Abstract:AIM To explore the effects of insulin-like growth factor-1(IGF1)on the metabolism of articular chondrocytes and their matrix.METHODS Monolayer cells of3-genera-tion obtained from4-week rabbit were cultured in DMEM containing10%fetal calf serum and were treated with IGF1 with doses ranging from1to100μg L-1 over2d ......