关键词: 人视网膜色素上皮细胞;微波;基因

    摘 要:目的 研究微波辐射与未辐射人视网膜色素上皮细胞(hTERT-RPE1)应激和凋亡相关基因转录的差异. 方法 应用基因芯片技术对2450MHz微波辐射、未辐射的视网膜色素上皮细胞的mRNA进行检测. 结果 在101条有关应激和凋亡的目的基因中发现有3条基因转录增加，有2条基因转录降低. 结论 微波辐射可引起细胞中与应激和凋亡有关的基因中多基因的改变.

    Study of microwaves radiation effects on the gene transcription profile changes in human retina pig┐ment epithelial cells

    LIU Xiu-Hong SHEN Hong SHI Yong-Liang CHEN Jing-Yuan CHEN Yao-Ming JI Ai-Ling

    1 Department of Environmental and Labor Hygiene，Faculty of Preventive Medicine，Fourth Military Medical University，Xi'an710033，China，

    2 Department of Pathology，First Military Medical University，Guangzhou510515，China

    Keywords:hTERT-RPE1;microwave;genes

    Abstract:AIM To study the difference of gene transcription between hTERT-RPE1cells radiated by microwaves and hTERT-RPE1cells about stress and apoptosis related genes.METHODS cDNA microarray was used to detect the mR-NA from hTERT-RPE1cells and the cells radiated by mic-rowaves.RESULTS Among the101aim genes，there were5genes with differences，of which，3genes displayed increa-sing transcription，and2genes decreasing transcription.CONCLUSION Microwave could induce the gene transcrip-tion profile changes in hTERT-RPE1cells about stress and apoptosis response genes. ......