聚合酶链反应_____cbl;_聚合酶链反应;

人cbl 基因真核表达载体的构建及表达

http://www.100md.com 《第四军医大学学报》 2004年第24期

聚合酶链反应,,cbl;,聚合酶链反应;,克隆，分子;,基因表达,人cbl基因真核表达载体的构建及表达,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Construction of eukaryotic expressing vector of human cbl gene and its expression

    LI Xia, ZHANG Jing, SU Jin, WANG LiFeng, LIU XinPing, LIU YunCai, YAO LiBo

    1Department of Biochemistry & Molecular Biology, School of Basic Medicine, Fourth Military Medical University, Xian 710033, China,2La Jolla Institute for Allergy & Immunology, San Diego, California 92121, USA

    【Abstract】AIM： To construct the eukaryotic expressing vector of human cbl and test its expression in African green monkey kidney cell line COS7. METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cloned into pGEMT easy, sequenced and then subcloned into eukaryotic expressing vector pcDNA3.1(+). The recombined vector was then transfected into COS7 cells with lipofectin and the expression of cbl gene was detected by Western blot. RESULTS: The eukaryotic expressing vector encoding human cbl was successfully constructed and its expression in COS7 cells could be detected. CONCLUSION: The eukaryotic expressing vector encoding human cbl is constructed and it expresses flagcbl fused protein correctly in COS-7 cells. ......

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