关键词: 原癌基因蛋白质p21(ras);转染;脱噬作用;肾肿瘤;肿瘤细胞，培养的

    摘 要:目的 探讨p21WAF1/CIP1 基因在顺铂诱导的GRC-1肾癌细胞凋亡过程中的作用. 方法 使用脂质体转染方法将正义p21WAF1/CIP1 基因及反义p21WAF1/CIP1 基因分别导入GRC-1肾癌细胞系中，对所获转染细胞进行形态学观察和流式细胞仪检测. 结果 在正义p21WAF1/CIP1 基因转染后GRC-1肾癌细胞中，顺铂诱导细胞凋亡的作用增强;在反义p21WAF1/CIP1 基因转染后GRC-1肾癌细胞中，顺铂诱导细胞凋亡的作用受到抑制. 结论 在GRC-1肾癌细胞中，顺铂诱导的细胞凋亡至少部分是通过p21WAF1/CIP1 基因发挥作用.p21WAF1/CIP1 基因作为一个与细胞凋亡的相关基因可能参与肾癌细胞凋亡的调控.

    Effect antisense p21WAF1/CIP1 gene on renal carcinoma cell apoptosis

    LIU He-Liang CUI Da-Xiang WANG He CHEN Bao-Qi SHAO Guo-Xing YAN Quan-Jian

    1 Department of Urolog Surgery，Xijing Hospital，

    2 Institute of Gene Diagnosis of Chinese PLA，Fourth Military Medical University，Xi'an710033，China

    Keywords:proto-oncogene protein p21(ras);transfection;apoptosis;kidney neoplasms;tumor cells，cul-tured

    Abstract:AIM To explore effect of p21WAF1/CIP1 gene on GRC-1renal carcinoma cell apoptosis mediated by Cisplatin.METHODS p21WAF1/CIP1 and antisense p21WAF1/CIP1 were re-spectively transfected into GRC-1renal carcinoma cell line by using lipofectin method.The observation of morphology and the detection of flow cytometer were made on transfected GRC-1cells.RESULTS Apoptosis mediated by Cisplatin were reinforced on p21WAF1/CIP1 gene transfected GRC-1cells ......