碘放射性同位素__(期刊论文)

125I-RC160的 NBS标记法研究及体内生物学分布特征

http://www.100md.com 《第四军医大学学报》 2004年第24期

碘放射性同位素,,碘放射性同位素；生长抑素类似物；同位素标记,125I-RC160的NBS标记法研究及体内生物学分布特征,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Study on preparation of 125I-RC160 with NBS and its biodistribution in animals

    WANG Zhe，WANG Jing，CHENG ShiWu，LI GuoQuan

    Department of Nuclear Medicine, Xijing Hospital, Fourth Military Medical University, Xian 710033, China

    【Abstract】AIM： To develop a high efficiency method for directly labeling RC-160 with 125I and to study its biodistribution in normal mice. METHODS: Using Nbromosuccinimide (NBS) as oxidant, rations of RC160 with variable 125I and NBS were added into the reaction system to find the optimal ratio of labeling response. The radioactivity biodistribution was performed in normal mice. RESULTS: The optimal ratio of RC160 ∶125I∶NBS was 10 μg∶7.4 MBq∶30 μg, the labeling efficiency was 76.9% and the radiochemical purity was 95.7% after purification by SepPak C18. 125I-RC-160 was eliminated fast from blood, heart and kidney. It was mainly concentrated in lung, liver, stomach and colon. CONCLUSION: This is an optimal method with simple steps and high labeling efficiency in labeling RC160 with 125I. 125IRC160 is mainly metabolized in digestive system. ......

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