关键词: Stathmin;原位杂交;成骨肉瘤

    摘 要:目的 明确stathmin基因在人成骨肉瘤组织有高表达并对其进行组织学定位. 方法 用地高辛标记全长stath-min基因，原位杂交方法检测stathmin基因在45例人成骨肉瘤组织及10例正常组织中的表达情况. 结果 在45例成骨肉瘤组织中有24例stathmin基因呈阳性表达(阳性率53%)，阳性颗粒可见于胞质及胞核;10例正常组织中2例呈弱阳性表达，差异显著(P<0.05). 结论 Stathmin基因在骨肉瘤中的高表达与骨肉瘤的发生、发展有重要的相关性，该基因有望成为人成骨肉瘤生物治疗中的重要靶基因.

    Expression of stathmin gene in osteosarcoma by in situ hybridization

    QU Jian-Bo，ZHANG Hui-Zhong，MA Bao-An，FAN Qing-Yu，ZHANG Zheng-Xu

    Institute of Orthopaedics Oncology of Chinese PLA，Tangdu Hospital，Fourth Military Medical Univer-sity，Xi'an710038，China

    Keywords:stathmin;in situ hybridization;osteosarcoma

    Abstract:AIM To observe over expression of stathmin gene and its localization in the tissue of osteosarcoma.METHODS The over expression of the stathmin gene was determined in45osteosarcoma specimens by in situ hybridiza-tion with Digoxigenin labled full length stathmin gene which were amplified from K562cells by RT-PCR method.RESULTS Over expression of stathmin gene was observed in24cases of osteosarcoma tissue(positive rate ......