[摘 要] 目的 应用Ames试验检测系统观察硫芥的诱变活性。 方法 选用TA97，TA98，TA100，TA102标准测试菌株，采用标准平板掺入法检测硫芥的诱变活性。加S9混合液作为体外代谢活化系统。结果 硫芥在S9活化和非活化两种测试条件下，8~500μg/皿浓度范围内，诱发TA98、TA100两种测试菌株回变菌落数与阴性对照组相比无明显增加，在8~32μg /皿浓度范围内，诱发TA97和TA102产生的回变菌落数与阴性对照相比无明显增加，在125~500μg /皿测试浓度范围内，对TA97和TA102产生的回变菌落数与阴性对照相比均达到阴性对照两倍以上。结论 硫芥具有碱基置换及移码型的直接诱变特性和间接诱变特性，间接诱变活性较直接诱变活性减弱。

    [关键词]：硫芥；Ames试验；突变

    Study on mutagenicity of sulfur mustard by Ames test

    Li Qi-hui, Dong Zhao-jun

    (Department of Military Toxicology, Third Medical University, Chongqing 400038, China)

    Abstract: Objective The mutagenicity of sulfur mustard was observed by Ames test detection system. Methods The TA97, TA98, TA100 and TA102 standard tester strains were used to detect the mutagenicity of sulfur mustard by using standard plate incorporation version. Adding S9 mixture to the plate for the use of metabolic activation system in vitro. Results With and without S9 activation, there is no obvious increase of the reverse strain clones in both TA98 and TA100 strains from 8~500μg /plate concentration, compared with negative group. The TA97 and the TA102 strains had the same reaction at concentrations of 8~32μg /plate. The reverse strain clones induced by sulfur mustard in TA97 and TA102 strain were twice as much as negative control group at concentrations of 125~500μg /plate. Conclusion Sulfur mustard was characterized by the direct and indirect mutagenicity with substitution mutant and frameshift mutant. Indirect mutagenicity is weaker than direct ones. ......