摘要 目的 探讨大鼠腹腔心脏移植模型手术技术的改进。方法 采用Ono创建的大鼠异位心脏移植模型，即将供体心脏主动脉和肺动脉分别与受体腹主动脉和下腔静脉吻合,对手术方法作了部分改进，缩短手术时间和供心缺血时间。结果 50次实验手术成功率88%。结论 新技术的采用降低了大鼠腹腔心脏移植模型的制作难度，手术失败的主要原因是吻合口出血，减少术中出血是保证手术成功的关键，吻合血管时应遵守“宁慢勿漏”的原则。

    [关键词] 大鼠；心脏移植

    Improved technique of heterotopic heart transplantation model in rats

    JIN Zhong-da, CHEN Jiang-hua,

    ( Nephropathy Center of the First Affiliated Hospital of Medical College of Zhejiang University, Hangzhou 310003, China)

    Abstract: Objective To develop a model of rat heterotopic heart transplantation by using improved techniques. Methods The authors adopted the model of rats heart heterotopic transplantation established by Ono who anastomosed the donor ascending aorta and pulmonary artery to the recipiet abdominal aorta and inferior vena cava respectively, and some improvements were made in this experiment. The time of procedure and the ischemic time of the donor heart were shortened. Results The successful rate of 50 formal transplantations was 88%. Conclusion Heterotopic heart transplantation model in rats is a new, simple and useful technique. The main reason of surgical failure is bleeding from the anastomosis. To lessen the intraoperative bleeding is a key factor to make the procedure successfully. ......