阿里红多糖对氧自由基的清除作用
超氧化物岐化酶,,阿里红多糖；自由基；超氧化物岐化酶；丙二醛；脂质过氧化,1材料与方法,2结果,3讨论,参考文献:
(新疆医科大学药学院药理教研室，天然药物与生药学教研室， 新疆乌鲁木齐830054)摘要：目的：研究阿里红多糖对氧自由基的清除作用。方法：采用现代生物化学发光技术，检测阿里红多糖对O2、H2O2的清除作用及对多种活性氧的总和清除率。采用黄嘌呤氧化酶法及硫代巴比妥酸(TBA)法测定小鼠心、肝、肾、脾等组织中SOD的活性及MDA的含量。结果：在体外阿里红多糖可有效的清除O2，其IC50为3.866×10-6(g/ml)；对H2O2的清除作用很弱，但能抑制人血嗜中性粒细胞“呼吸爆发”产生的多种自由基，IC50为1.668×10-3(g/ml)；阿里多红糖(20、40、80 mg/kg)可明显增加小鼠心脏和肝脏中SOD的活性，其中剂量为40、80 mg/kg的阿里红多糖可明显增加肝脏中SOD的活性，同时各给药组均可明显减少小鼠心脏、肝脏、肾脏和脾中MDA的含量，与对照组相比较差异有统计学意义(P<0.05)。结论：阿里红多糖有一定的清除氧自由基作用。
关键词：阿里红多糖；自由基；超氧化物岐化酶；丙二醛；脂质过氧化
Scavenging activity of fomes officinalis polysaccharides on oxygen free radicals
Ibadet·Tuohuti, Subat·Turdi, Maynur·Tuerxun, et al
(Department of Pharmacology， Xinjiang Medical University, Urumqi 830054，China)
Abstract: Objective: To investigate the scavenging effects of fomes officinalis polysaccharides (FOPS) on oxygen free radicals. Methods: Chemiluminescence methods were used to assess scavenging effects of FOPS on the O2, H2O2 and other oxygen free radicals. Thiobarbituric acid (TBA) and xanthine oxidase methods were used to assay superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in myocardial tissue, liver, kidney and spleen in mice, respectively. Results: FOPS had inhibitory effect on O2 production in vitro, having a 50% of inhibitory concentration (IC50) of 3.866×10-6 g/ml, and a weak inhibitory effect on H2O2 production with a IC50 of l.668×10-3 g/ml but showed a potent inhibitory effect on the various free radicals produced by “respiratory burst” of human leukocyte. FOPS (20, 40, 80 mg/kg) increased the SOD activity in myocardial tissue, liver and kidney of mice and decreased the MDA content of myocardial tissue, liver, kidney and spleen of mice. Conclusion: FOPS had scavenging effects on oxygen free radicals. ......
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