Role of nitric oxide in pantoprazole¬ðs protective effect from gastric mucosal lesion in rats

    LE Qiaoª²Liang£¬LIN Keª²Rong£¬ZHANG Zhiª²Jian

    Department of Gastroenterology, Fuzhou General Hospital, Nanjing Military Area Command, Fuzhou 350025, China

    ¡¾Abstract¡¿ AIM: To observe the role of nitric oxide(NO) in the protective effect of pantoprazole (PZ) on gastric mucosal lesion in rats. METHODS: Before gastric mucosal lesion was made in rats by pure alcohol, PZ(20 mg/kg), N¦Øª²nitroª²Lª²arginine methyl ester(Lª²NAME£¬4 mg/kg), Lª²arginine(250 mg/kg) and Dª²arginine(250 mg/kg) were injected into the vein. Gastric mucosal blood flow(GMBF) was assessed with laser Doppler flowmetry(LDF), gastric mucosal and serum NO-2/NO-3 were measured by cadmium granulation reduction and colorimetric method and the changes of ulcer index and the severity of tissue necrosis and neutrophils infiltration were observed. RESULTS: Ulcer index in the PZ group was much lower than that in the control group(P<0.01) and the degree of tissue necrosis and neutrophils infiltration were much milder than those in the control group(P<0.01). The protective effect of PZ was significantly decreased by prior administration of Lª²NAME. The inhibitory effect of Lª²NAME was antagonized by prior administration of Lª²arginine but not by Dª²arginine. PZ administered into the vein obviously increased GMBF, gastric mucosal and serum NO-2/NO-3, which was prevented by pretreatment with Lª²NAME but the antisecretory effect of PZ was not affected by Lª²NAME. CONCLUSION: PZ has some NO mediated protective effect against gastric mucosal lesion in rats and the action of PZ against gastric acid secretion contributes little to the protective effect. ......