Effect of mitomycin C polybutylcyanoacrylate magnetic nanoparticles on human BELª²7402 hepatoma cells transplanted BALB/c nude mice

    REN Fei, CHEN Jianª²Hai, CHEN Zhiª²Liang, X¦a Jun

    Department of Pharmacy Nanfang Hospital, Southern Medical University, Guangzhou 510515, China

    ¡¾Abstract¡¿AIM: To study the targeting therapeutic effect of mitomycin C polybutylcyanoacrylate magnetic nanoparticles (MMCª²PBCAª²MNPS) on nude mice transplanted subcutaneously by Belª²7402 cell lines. METHODS: Thirty nude mice transplanted subcutaneously by Belª²7402 cell lines were randomly divided into six groups (n=5). Each group was given, through tail vein, mitomycin normal saline (MMC), MMCª²PBCAª²MNPS and saline as control respectively. The groups of MMCª²PBCAª²MNPS were divided into magnetic field and nonmagnetic field. Tumors were moved and weighted to calculate the ratio of tumor inhibition at the end of the treatment. RESULTS: Under the influence of an external magnetic field of 4950Gs close to tumors in vitro, the inhibition rate of tumor weight growth by MMCª²PBCAª²MNPS was 73.9% (low dosage), 84.2% (moderate dosage) and 88.6% (high dosage), respectively. The inhibition rate of tumor growth by MMCª²PBCAª²MNPS was 54.0% (moderate dosage) in nonmagnetic field, higher than that of MMC normal saline group(27.6%, P<0.05). The pathological examination confirmed the aboveª²mentioned results. CONCLUSION: MMCª²PBCAª²MNPS with an external magnetic field significantly increases the antiª²tumor effects of MMC and decreases its side effects and cytotoxicity. ......