Experimental study of benzhydroxamic acid derivative in suppressing the activity of gastric cancer cell line in vitro

    ZHAO Changª²Hong, ZHANG Fengª²Min, SUI Guangª²Jie

    Tumor Hospital, Harbin Medical University, Harbin 150040, China

    ¡¾Abstract¡¿ AIM: To study the inhibitory action of derivative of benzhydroxamic acid on gastric cancer cell line and the mechanism. METHODS: Benzhydroxamic acid derivative E (medicine E) was artificially synthesized. The gastric cancer line 7901 was selected to serve as the target cell. The effect of medicine E on tumor cell activity was examined by cell culture technology in vitro. The change of morphology and the ultrastructure after treatment with medicine E was observed with the optic microscope and the electron microscope. The effect mechanism of medicine E was studied with immunohistochemistry and the PCR method. RESULTS: The gastric cancer cell activity was inhibited after treatment with medicine E. The cell number decreased to 54.55%, 82.05%, 94.85% and 94.97% after the cells were treated with medicine E for 1 d to 4 d, respectively. There was no live cells after the cells were treated with medicine E for 5 d and 6 d. The live cell number of medicine E group was significant reduced compared with that of blank control at different testing time points (1 d: t=4.24, P=0.005; 2 d: t=8.99, P=0.000; 3 d: t=13.36, P=0.000; 4 d: t=17.59, P=0.000; 5 d: t=31.00, P=0.000; 6 d: t=22.52, P=0.000). The tumor cellular shape changed after treatment. The survivin gene was suppressed after gastric cancer cells were treated with medicine E. CONCLUSION: The medicine E has inhibitory action on gastric cancer 7901 cells. The inhibitory action was the same or similar compared with that of 5ª²FU and DDP. The tumor cell apoptosis and growth suppression are caused mainly by suppression of the survivin gene expression after treatment with medicine E. ......