Role of Helicobacter pylori in development of gastric cancer

    TONG Shuª²Juan, LIU Yaª²Ping, YANG Danª²Dan

    1Basic Medical College, Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China, 2Department of Pathology, PLA No. 5 Hospital, Yinchuan 750004, China

    ¡¾Abstract¡¿ AIM: To study the possible carcinogenesis of Helicobacter pylori(Hp) infection in the development of gastric cancer. METHODS: Hp was determined by Warthinª²Starry silver staining in 39 samples of gastric cancer tissue, 24 samples of gastric mucosal atypical hyperplasia tissue and 33 samples of chronic gastritis tissue. HpcagA was determined by polymerase chain reaction (PCR) and the expressions of iNOS, VEGF, p185 and p21 were examined by Sª²P immunohistochemical staining. RESULTS: The expressions of Hp, HpcagA, iNOS, VEGF, p185 and p21 in gastric mucosal atypical hyperplasia and gastric cancer tissue were significantly higher than those in chronic gastritis. The expressions of iNOS, VEGF and p21 in Hp positive chronic gastritis, gastric mucosal atypical hyperplasia and gastric cancer cases were significantly higher than those in Hp negative cases. The expression of p185 in Hp positive gastric mucosal atypical hyperplasia and gastric cancer cases was significantly higher than that in Hp negative cases. The expression of Hp was positively related with iNOS, VEGF and p21 in chronic gastritis, gastric mucosal atypical hyperplasia and gastric cancer cases. The expression of Hp was positively related with p185 in gastric mucosal atypical hyperplasia and gastric cancer cases. CONCLUSION: Hp infection leads to the overexpression of HpcagA, iNOS and VEGF, which can stimulate the higher expressions of oncogene cª²erbBª²2 and ras. It may be one of the crucial mechanisms of induction of gastric cancer by Hp. ......