【摘要】 目的 探讨儿童支气管哮喘与亚甲基四氢叶酸还原酶(MTHFR)基因C677位点C→T多态性的关系。 方法 采用PCR和限制性内切酶片段长度多态性的方法对113例儿童支气管哮喘和131例健康儿童的MTHFR基因第677位点基因多态性进行研究。 结果 儿童支气管哮喘患者与对照组等位基因比较差异有显著性(χ 2 =6.69，P<0.05)。CC、CT、TT3种基因型之间差异存在显著性(χ 2 =6.35，P<0.05)。 结论 MTHFR C677位点C→T的基因突变可能与儿童支气管哮喘的发生有关。

    【关键词】 哮喘;亚甲基四氢叶酸脱氢酶;基因

    A study on MTHFR polymorphism and bronchial asthma in children

    LI Bo，HAN Hong-zhi，LI Shan-yu，SHI Jie-ping，HE Hai-tao，KONG Ning，LIU Ya .

    1.School of Public Health，Jilin University，Changchun 130021，China;2.The First Hospital of Jilin University，Changchun 130021，China;3.School of Pharmacy，Jilin University，Changchun 130021，China

    【Abstract】 Objective To investigate the association between bronchial asthma and MTHFR gene(methylenetetrahydrofolate reductase gene)polymorphism caused by the point mutation of cDNA677C to T.Methods Polymerase chain reaction and restriction fragment length polymorphism(PCR-RFLP)were used to detect MTHFR gene polymorphism in113patients with bronchial asthma and131healthy individuals.Results There was a significant difference in the frequency distribution of allele C and T between cases and controls(χ 2 =6.69 ......