Apoptosis of mouse myeloma Sp2/0 cells induced by supernatant of mouse cardiac muscle lysate

    WANG Wei, ZHANG Wenª²Yan, WU Jianª²Jun, LEI Yanª²Hua, JIN Jing, FANG Caoª²Hui, HU Yong, WANG Mingª²Li

    Department of Microbiology and Parasitology, Anhui Medical University, Hefei 230032, China

    ¡¾Abstract¡¿ AIM: To study the roles of mouse cardiac muscle lysate supernatant in inducing the apoptosis of mouse myeloma Sp2/0 cells. METHODS: Various concentrations (1¡Ã6, 1¡Ã12, 1¡Ã24) of mouse cardiac muscle lysate supernatant and cyclophosphamide were added into the Sp2/0 cell culture wells. After 24 h, microscope was used to observe the morphological changes of Sp2/0 cells and apoptosis rate was determined by flow cytometry after 48 h. Morphological changes of Sp2/0 cells coª²cultured with mouse cardiac muscle cells were observed after 10 h. The apoptosis of Sp2/0, Hela, Hepª²2 and Vero cells was induced with various concentrations (1¡Ã5, 1¡Ã10, 1¡Ã20 and 1¡Ã40) of mouse cardiac muscle lysate supernatant, liver lysate supernatant, thymus lysate supernatant and cyclophosphamide, and then the difference of apoptotic rate among them was compared. RESULTS: Mouse cardiac muscle lysate supernatant could induce the apoptosis of the Sp2/0 cells and the apoptotic rate increased in a concentrationª²dependent manner, but there was no difference between mouse cardiac muscle lysate supernatant group and cyclophosphamide group. After a 10ª²hour coª²culture with mouse cardiac muscle cell lysate, the apoptosis of a few Sp2/0 cells was seen. The mouse cardiac muscle lysate supernatant, liver lysate supernatant and thymus lysate supernatant could all induce apoptosis of the Sp2/0, HeLa, Hepª²2 and Vero cells. But the effect of the mouse cardiac muscle lysate supernatant was more obvious and permanent. CONCLUSION: The mouse cardiac muscle lysate supernatant can significantly induce apoptosis of mouse myeloma Sp2/0 cells. ......