实时荧光RTPCR对SARS患者血清中病毒含量的定量检测
SARS病毒,,SARS病毒;逆转录聚合酶链反应;定量分析;血清,0引言,1材料和方法,2结果,3讨论,【参考文献】
Realtime fluorescent RTPCR quantitative detection of SARS CoV in serum of infected patientsLI ZhenYong, SHAO DaXiao, FENG YanMing,LI ZhiTao, JING JianZhou2,WANG YunLong, QU LingBo, ZHAO YuFen
1Key Laboratory of Chemobiology, Faculty of Chemistry, Zhengzhou University, Zhengzhou 450052, China, 2Henan Provincial Research Centre of Biotechnology, Zhengzhou 450056, China
【Abstract】 AIM: To detect SARSCoV in various clinical specimens such as sputum, gargling liquid, throat swab and stool and explore the association between the disease course and the SARSCoV content. METHODS: We quantitatively detected 156 serum specimens from SARS patients by real time RTPCR. The viral load in SARSCoV positive serum was analyzed using the coronavirus deactivated with 9.4×109 copies/L. Robert Koch Institute as standard preparation and viruslike particles as positive controls. RESULTS: The sensitivity of the detected agent was 1×105 copies/L. Eightyone of the 156 cases (51.9%) tested were positive with the viral load ranging from 1×105-1×107 copies/L. The viral load in serum samples was generally low as compared with data reported for other specimens. CONCLUSION: There is no significant relationship between the low viral load and the disease course. ......
您现在查看是摘要页,全文长 9079 字符。