Regulation of estradiol and its receptor antagonist on ovarian cancer cell secreting and synthesizing interleukinª²6

    LU Lei, XIN Xiaoª²Yan, MA Xiangª²Dong, SHAN Li, YU Yueª²Cheng, GUO Huiª²Ling

    1Department of Obstetrics & Gynecology, Xijing Hospital, Fourth Military Medical University, Xi¬ðan 710033, China, 2Department of Obstetrics & Gynecology, Shaanxi Provincial Corps Hospital, Chinese People¬ðs Armed Police Forces, Xi¬ðan 710054, China

    ¡¾Abstract¡¿ AIM: To discuss estradiol in the regulation of interleukinª²6 (ILª²6) excretion and synthesis of ovarian cancer cell HOª²8910. METHODS: Different concentrations of estradiol (E2) and its receptor antagonist were used to affect HOª²8910 in vitro. ILª²6 content of cell culture supernatant was detected by ELISA. ILª²6 and the localization and dose change in cells were examined by immunohistoª²chemical method. RESULTS: 1¡Á10-6 , 1¡Á10-8 and 1¡Á10-10 mol/L E2 were coª²cultivated with cells for 3 d and the concentration of ILª²6 in cell culture supernatant increased by 93.42%, 82.89% and 22.37% respectively. They were significantly higher than those in controls (P<0.05). Immunohistoª²chemical staining showed that ILª²6 was expressed in nuclear membrane. The group using E2 receptor antagonist ICI 182780 had no difference with the controls. CONCLUSION: E2 binding with its receptor accelerates the ovarian cancer cell HOª²8910 to synthesize and secrete ILª²6, which may increase the risk of hormone replacement therapy in ovarian cancer patients after operation. ......