Establishment of orthotopic model of hamster pancreatic cancer and its biologic characteristics

    BAI Jiª²Gang, L¦a Yi, WANG Haoª²Hua

    Department of Hepatobiliary Surgery, First Hospital, Xi¬ðan Jiaotong University, Xi¬ðan 710061, China

    ¡¾Abstract¡¿AIM: To establish an orthotopic model of hamster pancreatic cancer and to analyze its biologic characteristics. METHODS: Pancreatic cancer cell line pDHAMª²1 of hamster was inoculated under pancreatic membrane. At 7th, 14th, 21st, and 28th day, a group of 10 animals were sacrificed to observe the growth of orthotopic cancer, lymph nodes metastasis, liver metastasis and the quantity and quality of ascites. RESULTS: Orthotopic model of hamster pancreatic cancer had a high survival rate (95%) and grew rapidly. The orthotopic tumors were measured (16¡À6), (173¡À44), (369¡À87), (974¡À226) mm3 respectively at the 7th, 14th, 21st and 28th day. At the 21st day, cancer cells were observed in lymphatic capillaries and metastasis in some lymph nodes and hydremic ascites was detected. At the 28th day, the positivity rate of lymph nodes and the amount of bloody ascites increased. Liver metastasis was detected in some of the hamsters. CONCLUSION: The establishment of orthotopic model of hamster pancreatic cancer cells pGHAMª²1 is simple and easily copied, and maintains the biologic characteristics of pancreatic carcinoma, which is an ideal research model of pancreatic carcinoma. ......