Preparation and characteristic test of bFGFª²impregnated microsphere

    LIU Rui, HUANG Sha, JIN Yan, WU Hong, JIANG Ling, LIU Tao

    1Team 10, Cadet Brigade, 2Department of Oral Histology and Pathology, College of Stomatology, 3Department of Chemistry, School of Pharmacy, 4Tissue Engineering Technology Center, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To prepare the basic fibroblast growth factor (bFGF)ª²impregnated microsphere and to study its characteristics. METHODS: The bFGFª²impregnated microsphere was prepared by improved emulsified coldª²condensation method. The physical properties, drug content and encapsulation were detected. The drug release in vitro was measured by ELISA. RESULTS: The average diameter of microsphere was (12.4¡À3.6) ¦Ìm, the average drug content was 0.24% and encapsulation was 96.82%. In vitro, 28.23% of bFGF was sustainedª²released on the first day, 57.19% from the second to the fifth day and 93.94% by the seventh day. CONCLUSION: The preparation method is simple, rapid and accurate and the release of bFGF from the microspheres can be sustained for a longer time. ......