蛋白酶激活受体__(期刊论文)

人肺上皮细胞表达蛋白酶激活受体

http://www.100md.com 《第四军医大学学报》 2005年第17期

蛋白酶激活受体,,蛋白酶激活受体；肺上皮细胞；RTPCR；流式细胞术；免疫荧光细胞染色,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Expression of protease activated receptors on human lung epithelial cells

    WANG HaiYan, HE ShaoHeng, FU YiLing, FANG ZeMan

    Allergy and Inflammation Research Institute, Shantou University Medical College, Shantou 515031, China

    【Abstract】 AIM: To explore the expression of protease activated receptors (PARs) on lung epithelial cells. METHODS: We used RTPCR, flow cytometry and immunofluorescence cell staining techniques to observe the expression of PARs on A549 cells. RESULTS: All 4 PARs (PAR1, PAR2, PAR3 and PAR4) were expressed on A549 cells. CONCLUSION: PAR1-4 are expressed on human lung epithelial cells, which may facilitate further investigation of the potential functions of PARs on lung epithelial cells.

    【Keywords】 protease activated receptors；lung epithelial cell; RTPCR; flowcytometry; immunofluorescence cell staining

    【摘要】目的: 蛋白酶激活受体(proteaseactivated receptors, PARs) 广泛分布在多种组织细胞上，但4种PARs在肺上皮细胞的表达情况尚不清楚. 我们用A549细胞研究PARs在肺上皮细胞的表达. 方法:采用逆转录聚合酶链反应(RTPCR)、流式细胞仪和免疫荧光细胞染色分析技术分析肺上皮细胞系A549细胞PARs的表达情况. 结果:4种PARs在肺上皮细胞上均有不同程度的表达. 结论:肺上皮细胞同时表达PAR 1~4 4种受体 ......

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