Effects of retinoids on proliferation of human melanoma cell line A375

    NIU Xinª²Wu, FENG Jie, PENG Zhenª²Hui, MA Huiª²Qun, LIU Chao, ZHANG Xianª²Qi

    Department of Dermatology, Second Hospital of Xi¬ðan Jiaotong University, Xi¬ðan 71004, China

    ¡¾Abstract¡¿ AIM: To investigate the effects of diverse retinoids and receptor agonists in inhibiting proliferation of human melanoma cell line A375. METHODS: Human melanoma cell line A375 was treated by diverse retinoids (atª²RA, 13ª²cis RA and 9ª²cis RA), MA (RXR agonist) and TTNPB (RAR agonist) and their effects on the proliferation and cell cycle of A375 cells were analyzed by MTT and flow cytometer. RESULTS: Both retinoids and TTNPB inhibited the proliferation of A375 cells, but MA had no such effect. At all concentrations (10-5, 10-6 and10-7 mol/L) in 24 h and 10-5 mol/L in 48 and 72 h, TTNPB had the most powerful effect in all reagents (P<0.05). The results of cell cycle analysis showed that retinoids and TTNPB caused the blockage of G0/G1 phase, but MA had no such effect. At 10-5 mol/L in 24 and 48 h, TTNPB had the most powerful effect of causing blockage of G0/G1 phase in all reagents (P<0.05). CONCLUSION: Retinoids can inhibit the proliferation and cause the blockage of G0/G1 phase in human melanoma cell line A375. It is not the activation of RXR but the activation of RAR that may be related to these effects. ......