流式细胞术_____DNA_polβ;

不同类型的DNA聚合酶β基因对CHO细胞的影响

http://www.100md.com 《第四军医大学学报》 2005年第19期

流式细胞术,,DNA,polβ;,转染;,CHO细胞;,RTPCR;,流式细胞术,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Effect of wildtype and mutant DNA polymerase β expression on CHO cells growth

    ZHAO JiMin, JIN Ge, HUANG YouTian, YANG HongYan, ZHENG ZhiMin, DONG ZiMing

    1Department of Pathophysilogy, 2Department of Biochemistry, School of Basic Medical Sciences, Zhenzhou University, Zhenzhou 450052, China

    【Abstract】 AIM: To study the effects of the DNA polymerase β (DNA polβ) of wild type and the mutant(58 bp deletion) on Chinese hamster ovary cells (CHO). METHODS: The expression of mRNA of wild type and the mutant in transfectant cells was determined by RTPCR and the growth curve of transfected CHO cells was drawn with the MTT assay. The changes in cell cycle were analyzed by flow cytometry. RESULTS: Cell lines stably expressing wildtype and the mutational polβ were established. The CHO cells transfacted by the mutant gene were higher than untransfacted CHO cells in the growth rate of CHO cells and the percentage of S phase cells in vitro (P<0.05). The effects of the wildtype enzyme on growth rate and cell cycle were not observed(P>0.05). CONCLUSION: The wild type and the mutant have different effect on CHO cells. ......

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