人端粒酶催化亚单位基因正反义腺病毒表达载体的构建及鉴定
腺病毒,,人端粒酶催化亚单位;,腺病毒;表达载体,0引言,1材料和方法,2结果,3讨论,【参考文献】,[1]YangSM,FangDC
Construction and identification of sense and antisense human telomerase catalytic subunit adenovirus expression vectorCHEN Ling, YANG ShiMing, CAI YongGuo, FANG DianChun, LUO YuanHui
Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
【Abstract】 AIM: To construct an adenovirus expression vector of sense and antisense human telomerase reverse transcriptase (hTERT) gene. METHODS: The hTERT cDNA fragment contained in the pGRN145 vector was cloned into the adenovirus expression vector pDC315 in cisdirection or transdirection using DNA recombinant technology. The recombinant vectors were identified by digestion of BamH I and the sense and antisense recombinant vector was further identified by DNA sequencing. RESULTS: After digested by BamH I, two fragments which lengthened 1.0 and 6.4 kb were formed in sense recombinant vector (pDC315shTERT), while three fragments which lengthened 1.0, 2.5 and 3.9 kb were formed in antisense vector (pDC315ahTERT). Electrophoresis results were completely consistent with theoretical calculation. The DNA sequence measurement confirmed the linking direction of sense and antisense pDC315hTERT. CONCLUSION: The sense and antisense hTERT adenovirus expression vectors are successfully constructed. ......
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