巨细胞病毒_____巨细胞病毒,(期刊论文)

重组PP150抗原用于胶体金免疫斑点技术检测抗HCMVIgM

http://www.100md.com 《第四军医大学学报》 2005年第19期

巨细胞病毒,,巨细胞病毒,人；基因pp150;原核表达；胶体金;免疫斑点法,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Recombinant PP150 of human cytomegalovirus as antigene for colloidal gold immunodot assay to detect HCMVIgM in sera

    WANG Qing, WANG XiBo, XU LongQiang，YU WeiLing, YU Hong, ZHANG WenQing

    1Department of Clinical Laboratory, Affiliated Hospital, 2Department of Medicl Microbiology, Medical College, Qingdao University, Qingdao 266003, China

    【Abstract】 AIM: To use recombinant pp150 of human cytomegalovirus (HCMV) in colloidal gold immunodot assay (CGIDA) for the detection of HCMVIgM in sera. METHODS: A fragment of gene coding for pp150 antigen epitopes (840-1048aa) was expressed in E. coli and the fusion protein was purified. The results were compared with those of specific antibodies ELISA IgM. RESULTS: Recombinant antigen CGIDA had a good agreement (96.0%) with ELISA kit. The specificity of CGIDA was 100% and the sensitivity was 74.2%. CONCLUSION: Recombinant pp150 is an effective and valuable antigen used in CGIDA for detecting HCMVIgM.

    【Keywords】 cytomegalovirouses,human;gene pp150;prokaryotic expression; gold colloidal; immunodot assay

    【摘要】目的: 探索应用重组人巨细胞病毒(HCMV)PP150抗原用于免疫滴金技术检测血清中抗HCMVIgM的诊断价值. 方法: PCR扩增HCMV pp150抗原决定簇(840～1048aa)编码基因片段 ......

百拇医药网 http://www.100md.com/html/DirDu/2006/03/12/94/97/70.htm

您现在查看是摘要页，全文长 10331 字符。