Immunzition treatment of Helicobacter pylori infection with two valence vaccine composed of catalase and adhesin conservation: An animal model study

    BAI Yang1, CHEN Li, WANG Jiª²De, SHI Li, CHANG Shaoª²Hong, ZHANG Zhaoª²Shan3, ZHOU Dianª²Yuan, ZHANG Yaª²Li

    1PLA Institute for Digestive Diseases, Southern Medica1 University, Guangzhou 510515, China, 2Department of Infectious Diseases, Subei People¬ðs Hospital, Yangzhou 225002, China, 3Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China

    ¡¾Abstract¡¿ AIM: To investigate the effects of two valence vaccine consisting of Helicobacter pylori (H. pylori) catalase and adhesin conservation in the treatment of H. pylori infection in an H. pylori infected mouse model. METHODS: C57BL/6 mice infected with H. pylori were divided into seven groups. The mice of each group were immunized respectively by catalase and adhesin conservation (separately, 100 ¦Ìg) plus CT (2 ¦Ìg), catalase (100 ¦Ìg) plus CT (2 ¦Ìg), adhesin conservation (100 ¦Ìg) plus CT (2 ¦Ìg), catalase (100 ¦Ìg), adhesin conservation (100 ¦Ìg) and CT (2 ¦Ìg), PBS orally once a week for four weeks. Four weeks after the last treatment, all animals were sacrificed and stomach biopsies were collected to detect H. pylori by semiª²quantitative bacterial culture assay. RESULTS: The eradication rate of two valence vaccine group, single valence vaccine group of catalase and single valence vaccine group of adhesin conservation was 69.2%(18/26), 30.8%(8/26) and 38.5%(10/26), respectively. The eradication rate of the other four groups was all 0%. The H. pylori colonized density of the vaccination group was significantly lower than that of the other four groups (P<0.05). There were significantly differences in the eradication rate and H. pylori colonized density between the two valence vaccination group and the single valence vaccination groups (P<0.05). CONCLUSION: The two valence vaccine consisting of catalase, adhesion conservation and adjuvant has better immunotherapeutic effects than the single valence vaccine. More H. pylori vaccine research should be directed to the multiª²valence vaccine. ......