幽门螺杆菌重组VacA蛋黄抗体的体外活性
http://www.100md.com
毛小琴, 杨致邦, 张绍兰, 田一玲, 黄 进
幽门螺杆菌;细胞空泡毒素;重组蛋白;蛋黄抗体;活性;细胞毒性毛小琴,杨致邦,张绍兰,田一玲,黄进.幽门螺杆菌重组VacA蛋黄抗体的体外活性.世界华人消化杂志2006;14(8)789-794,幽门螺杆
 |
| 第2页 |
 |
| 第1页 |
参见附件(940KB，6页)。
毛小琴, 杨致邦, 张绍兰, 田一玲, 黄进,重庆医科大学基础医学院微生物学教研室, 重庆, 400016
毛小琴,2002年重庆医科大学硕士. 主要从事幽门螺旋杆菌感染的研究.
通讯作者:杨致邦, 400016, 重庆市, 重庆医科大学基础医学院微生物学教研室. dryangfm365@sina.com
电话:023-68485090 传真:023-68486859
收稿日期:2006-01-07 接受日期:2006-01-26
In vitro activity of IgY against recombinant VacA protein of Helicobacter pylori
Xiao-Qin Mao, Zhi-Bang Yang, Shao-Lan Zhang, Yi-Ling Tian, Jin Huang
Xiao-Qin Mao, Zhi-Bang Yang, Shao-Lan Zhang, Yi-Ling Tian, Jin Huang, Department of Microbiology, College of Basic Medicine, Chongqing University of Medical Sciences, Chongqing 400016, China
Correspondence to: Dr. Zhi-Bang Yang, Department of Microbiology, College of Basic Medicine, Chongqing University of Medical Sciences, Chongqing 400016,China. dryangfm365@sina.com
Received: 2006-01-07 Accepted: 2006-01-26
Abstract
AIM:To explore the physicochemical and biolo-gical properties of IgY extracted from the yolk of hen’s egg immunized with recombinant VacA protein of Helicobacter pylori.
METHODS:The purified antigen of recombinant VacA was used to immunize the hens and the VacA-IgY antibody was extracted from the yolk of hen’s egg by water dilution methods and then purified by deposition technique with ammonium sulfate. In order to evaluate VacA-IgY heat stability, the titer of VacA-IgY was detected by enzyme-linked immunosorbent assay (ELISA) after being heated for 15 min under different temperatures. VacA-IgY was added into Tris-HCl of different pH values and incubated under a temperature of 37℃ to access its acid stability using ELISA. The competence of anti-pepsin digestion was also measured by ELISA. The VacA+ H pylori were co-cultured with Hela cells and VacA-IgY antibody was added to observe its neutralization on the cytotoxity of H pylori by MTT assay in vitro.
RESULTS: The activity of VacA-IgY was maintained about 50% after water baths (70℃, 15 min) ......
您现在查看是摘要介绍页，详见PDF附件(940KB，6页)。