Role of OX34 in treatment of acute rejection after small bowel transplantation in rats

    JI Gang, LIN Yan, WANG Weiª²Zhong, GUAN Wenª²Xian, NIU Weiª²Bo

    Department of Gastrointestinal Surgery, Xijing Hospital, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To investigate the therapeutic effect of OX34 against acute rejection after small bowel transplantation (SBT) in rats. METHODS: A total of 72 rats of 2 inbred lines (SD and Wistar) were randomly divided into 4 groups(A, B, C and D). Group A (n=18) accepted fictitious operation and was fed with common forage; Group B (n=18) underwent isoª²strain SBT and the treatment of postª²operation such as routine fluid replacement and antibiotic administration; Group C (n=18) had anisoª²strain SBT and the same postª²operative treatment as group B; and Group D(n=18)got the same operation as group C and OX34 was added into the same postª²operative treatment as group B. Samples of grafts and peripheral blood were taken from 3, 5 and 7 d after SBT and were detected by pathology and flow cytometer. RESULTS: The survival time and the positive expression rate of CD2 in peripheral blood of group C were significantly different compared with those of the other 3 groups (P<0.05). Acute rejection developed from mild to severe when the samples were taken form 3, 5, 7 d after SBT. Part of the grafts from group D suffered from a mild degree of acute rejection 5 and 7 d after SBT, while none of the group A and B showed pathological evidence of acute rejection. CONCLUSION: OX34 significantly suppresses the acute rejection after SBT in vivo. ......