关键词: 细胞周期蛋白类;细胞周期蛋白质依赖激酶类;表皮生长因子-尿抑胃素;真皮;成纤维细胞

    摘 要:目的 探讨表皮生长因子(EGF)对作为皮肤组织工程种子细胞之一的真皮成纤维细胞中cyclinD1和CDK-4表达的影响，以从细胞周期探讨EGF促进真皮成纤维细胞生长的机制. 方法 用含100mL·L-1 胎牛血清的DMEM，加入50mg·L-1 的EGF培养SD大鼠的真皮成纤维细胞，通过MTT检测、流式细胞仪分析观察细胞的生长状态，免疫组化检测cyclinD1和CDK-4的表达，结合流式细胞仪分析观察细胞的周期变化. 结果 MTT检测、流式细胞仪结果都显示50mg·L-1 的EGF能显著促进真皮成纤维细胞的生长增殖，免疫组化结果显示，二者一致. 结论 50mg·L-1 的EGF对真皮成纤维细胞的生长有极大的促进作用，促进了细胞周期蛋白cyclinD1和CDK-4的表达，使细胞G1期变短.

    Effects of EGF on the cell cycle in dermal fibrob┐lasts of SD rats

    WANG Jun-Lin，LIU Yuan，JIN Yan，LU Hong-Bing，ZHAO Yu，WANG Xin-Wen，DONG Rui

    Department of Oral Histopathology，Stomatological College，Fourth Military Medical University，Xi'an710033，China

    Keywords:cyclins;cyclin-dependent kinases;epidermal growth factor-urogastrone;dermis;fibroblasts

    Abstract:AIM To examine the biological behaviors of der-mal fibroblasts in DMEM containing50mg·L-1 EGF and to investigate the expressions of cyclinD1and CDK-4.METH┐ODS Dermal fibroblasts were cultured in DMEM containing50mg·L-1 EGF and100mL·L-1 fetus bovine serum.The proliferation ability was investigated by MTT detection and FCM analysis.The expressions of cyclinD1and CDK-4were investigated by immunohistochemistry method.RESULTS Dermal fibroblasts cultured in DMEM containing50mg·L-1 EGF showed enhanced proliferation and more positive signals for cyclinD1and CDK-4.CONCLUSION The dermal fi-broblasts cultured in DMEM containing50mg·L-1 EGF were in better condition than those cultured in DMEM with-out EGF.And EGF shortened the G1phase of the cell cycle. ......