重组_____转化生长因子β1；重组融合蛋白

重组人TGFβ1融合蛋白的克隆、表达、纯化及鉴定

http://www.100md.com 《第四军医大学学报》 2006年第3期

重组,,转化生长因子β1；重组融合蛋白,分离与纯化；pGEX4T1载体,1材料和方法,2结果,3讨论,【参考文献】

     expression, purification and identification of recombinant human TGFβ1 fusion protein

    JIANG ChangLi, ZHANG YingQi, YAN Zhen

    Biotechnology Center, School of Pharmacy, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To clone, express and purify human transforming growth factor β1 (TGFβ1) in E.coli. METHODS: A 336 bp of human rhTGFβ1 gene fragment was obtained by RTPCR and cloned into pGEX4T1 vector, a GST fusion expression vector. The plasmid was transformed into E.coli DH5α and induced to express fusion protein GST rhTGFβ1 with IPTG. The expression of rhTGFβ1 was detected by SDSPAGE and Western Blot. RESULTS: A novel protein with expected molecular mass was expressed. The expressed product showed a good binding ability to antirhTGFβ1 monoclonal antibody. CONCLUSION: Our successful cloning and expression of rhTGFβ1 gene and purification of rhTGFβ1 protein lay a basis for the production of rhTGFβ1 autovaccine. ......

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