关键词: 激光;血管外科手术;动物实验替代实验

    摘 要:目的 应用线形CO2 激光器和血管支撑物进行小血管吻合，观察术后血管吻合口的远期通畅率、动脉瘤发生率及物理性质. 方法 家兔104只，随机分为8组，每只家兔的左颈动脉离断后用激光进行吻合，右颈动脉则用缝线进行吻合.在术后1h，24h，3d，1，2，4，8和12wk时分别再次麻醉动物，解剖颈动脉，观察血管通畅情况，并测试吻合口的耐压强度和抗拉强度. 结果 术后血管吻合口的即刻通畅率均为100%;远期通畅率，激光吻合组为98%，缝线吻合组为94%;动脉瘤发生率，激光组为3%，缝线组为0.耐压强度测定，术后1wk内激光组显著高于缝线组，2wk后两组之间无差异.抗拉强度测定，2wk内，激光组显著低于缝线组，4wk后两组间无差异. 结论 应用线形CO2 激光器和血管支撑物进行小血管的吻合是一项可行的技术，它在远期通畅率、动脉瘤发生率及血管物理性质几方面都取得了令人满意的结果.

    Experiment study of microvascular anastomosis with linearing CO2 laser

    ZHAO Rong YANG Ji-Qing CAI Zhen-Jie

    1 Institute of Cardiovascular Surgery，Xijing Hospi-tal，

    2 Department of Physics，Faculty of Biomedical Engineering，Fourth Military Medical University，Xi'an710033，China

    Keywords:lasers;vascular surgical procedures;animal test-ing alternatives

    Abstract:AIM To conduct laser-assisted microvascular anastomsis by using linearity CO2 laser and vascular suste-nance，and to observe the long-term patency rates，aneurysm rates and physical qualities of postoperation.METHODS 104rabbits were divided into8groups.Bilateral carotid ar-teries of rabbits were cut off ......