关键词: 肝/细胞学;连续传代培养;显微镜检查，电子;大鼠

    摘 要:目的 将大鼠肝细胞进行传代培养，并观察其形态学特点. 方法 采用胶原酶二步法灌注1mo龄SD雄性大鼠肝脏，制备肝细胞悬液并进行原代及传代培养，对其形态学进行了观察. 结果 肝细胞已传7代，每次传代培养6d左右，光镜下可见大量新生肝细胞生长，呈多边形，相嵌紧密排列.电镜下可见核变形，有的为双核细胞.此外，胞质内的糖原颗粒也逐渐减少. 结论 成功进行了肝细胞的传代培养，为肝细胞的研究提供了一种方便的研究方法.

    Morphological observation of rat subcultured hepa-tocytes

    ZHANG Meng CHEN Bi

    1 Department of Burn&Plastic Surgery，Xi'an Mu-nicipal Central Hospital，Xi'an710003，China，

    2 Department of Burn Surgery，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keyword:iiver/cytology;serial passage;microscopy，elec-tron;rats

    Abstract:AIM To subculture rat hepatocytes and to ob-serve their morphological characteristics.METHODS Hepa-tocytes were isolated from1-month-old rats by two-step col-lagen perfusion.After cultured for20d，the primary culture-sof hepatocytes were separated with trypsin and then subcul-tured for7generations whose morphological characteristics were studied.RESULTS Six days after the subculture，hep-atocytes were observed under light microscope ......