关键词: 哮喘;嗜酸粒细胞;嗜酸粒细胞趋化因子

    摘 要:目的 探讨过敏性哮喘中嗜酸粒细胞趋化因子Eo-taxin致气道炎症的机制. 方法 将豚鼠40只随机分为哮喘组和对照组，卵蛋白致敏及诱发过敏性哮喘.①收集支气管肺泡灌洗液(BALF)，观察细胞总数(TC)及嗜酸粒细胞(Eos)、巨噬细胞(AM)、淋巴细胞(L)、中性粒细胞(N)比例变化;②豚鼠肺组织病理标本经HE染色，观察病理学改变;③采用原位分子杂交技术观察肺组织中Eotaxin mRNA的表达;④采用Northern blot方法观察肺组织中Eotaxin mRNA表达情况. 结果 过敏性哮喘豚鼠BALF中TC，Eos比例较对照组明显升高(P<0.01)，AM比例显著下降(P<0.01)，L和N比例与对照组相差不明显(P>0.05).哮喘豚鼠肺组织中可见气道炎症反应及Eos和L粘膜下浸润.支气管粘膜、粘膜下层及周围肺组织中Eotaxin mRNA阳性细胞表达率(198±46)mm-2 较对照组(16±8)mm-2 明显增加(P<0.01).Nortthern blot杂交可见哮喘组表达Eotaxin mRNA较对照组明显升高. 结论 过敏性哮喘中Eotaxin基因表达明显增强，同时伴有Eos肺内募集及激活，进而导致气道炎症而诱发哮喘.

    Role of eotaxin in airway inflammation of allergic asthma

    YU Hua-Peng QI Hao-Wen CHEN Xin WANG Jun DENG Huo-Jin LIU Yu-Hua

    1 Department of Respiratory Diseases，Xijing Hospi-tal，Fourth Military Medical University，Xi'an710033，China，

    2 Department of Respiratory Diseases，Zhujiang Hospital，First Military Medical University，Guangzhou510282，China

    Keywords:asthma;eosinophil;eotaxin

    Abstract:AIM To study the role of eotaxin in the airway inflammation of asthma.METHODS ①Ovalbumin(Ova)sensitized and challenged guinea pigs;②Bronchial alveolar lavage fluid(BALF)of guinea pigs were collected ......