关键词: 工频磁场;MCF-7细胞;RT-PCR;p53

    摘 要:目的 观察工频磁场暴露对MCF-7细胞p53mR-NA表达的影响. 方法 将人乳腺癌MCF-7细胞暴露或假暴露于60Hz，5mT工频磁场中4，8和24h，采用RT-PCR方法定量分析照射后不同时间MCF-7细胞p53mRNA的表达水平. 结果 工频磁场暴露4，8和24h，MCF-7细胞p53mRNA的表达水平保持不变. 结论 工频磁场暴露对MCF-7细胞p53mRNA的表达没有影响.

    Analysis of p53mRNA expression in MCF┐7cells exposed to power┐frequency magnetic fields

    DING Gui-Rong GUO Guo-Zhen GUO Yao Miyakoshi Junji

    1 Department of Radiation Medicine，Faculty of Pre-ventive Medicine，Fourth Military Medical Univer-sity，Xi'an710033，China，

    2 Department of Radia-tion Genetics，Graduate School of Medicine，Kyoto University，Yoshida-Konoe-cho，Sakyo-ku，Kyoto606-8501，Japan

    Keywords:power-frequency magnetic field;MCF-7cells;RT-PCR;p53

    Abstract:AIM To observe effects of power-frequency mag-netic fields on p53mRNA expression in MCF-7cells.METHODS Human breast cancer MCF-7cells were exposed or sham-exposed to60Hz ......