关键词: TNP-470;Lovo细胞;脱噬作用;结肠肿瘤

    摘 要:目的 探讨TNP-470对体内外人结肠癌Lovo细胞生长的影响. 方法 采用MTT法检测TNP-470对体外培养的Lovo细胞的生长抑制作用.建立结肠癌皮下移植瘤模型，16只裸鼠平均分为治疗组(TNP-47030mg kg-1 )和对照组(相同体积的生理盐水)，4wk后处死裸鼠，测量肿瘤体积.肿瘤组织行病理学检查，采用HE染色和TUNEL原位末端标记术检测体内肿瘤细胞的凋亡情况. 结果 TNP-470作用72h，明显抑制体外培养的Lovo细胞的生长，IC50为55.8μg L-1 .TNP-470显著抑制了体内肿瘤的生长，治疗组肿瘤体积为(731±40)mm3 ，对照组为(1345±38)mm

    3 ，(P<0.01).治疗组Lovo细胞凋亡率为(21.4±2.1)%，对照组为(7.5±1.5)%，(P<0.001). 结论 TNP-470能够抑制结肠癌Lovo细胞的体内外生长，其机制可能与诱导细胞凋亡有关.

    Inhibitory effects of TNP┐470on growth of human colon cancer Lovo cells

    FAN Ying-Fang HUANG Zong-Hai NIE Jing

    1 Department of General Surgery，Zhujiang Hospi-tal，First Military Medical University，Guangzhou510282，China，

    2 Department of Surgery，Henghu Hospital，Nanchang330123，China

    Keywords:TNP-470;Lovo cells;apoptosis;colonic neo-plasms

    Abstract:AIM To study the effect of TNP-470on the growth of human colon cancer cell line，Lovo cells，in vitro and in vivo.METHODS Growth inhibitory effect of TNP-470on Lovo cells was examined by an MTT assay.Human colon cancer xenografts was transplanted into16nude mice.TNP-470(30mg/kg)or the same volume of saline solution was administered on alternative day starting1days after tu-mor implantation.Mice were sacrificed after4weeks and tu-mors were processed for histological examination.In both the treatment and control groups ......