关键词: 维生素C;维生素E;辐射损伤;抗氧化;超氧化物歧化酶;丙二醛

    摘 要:目的 探讨Vit.C，E对60 Coγ线照射家兔的保护作用以及剂量效应关系. 方法 中国本兔随机分为12组，每组5只.Vit.C5组iv，剂量分别为10，20，40，80和160mg·kg-1 ，Vit.E油剂ig5组，剂量分别为5，10，20，40和80mg·kg-1 .第3日进行60 Coγ线(4.5Gy)照射家兔.第5日处死、剖腹取肝脏和腹腔静脉血，分别测定超氧化物歧化酶(SOD)的催化活性和丙二醛(MDA)的含量变化. 结果 辐射动物补充Vit.C和Vit.E组的肝SOD的催化活性与阴性对照组相比明显升高(P<0.01)，Vit.E的作用更明显，呈现了一定剂量效应关系;各Vit.C组肝脏MDA的含量变化不显著(P>0.01)，但血清MDA的含量轻微下降;各Vit.E组肝脏和血清中的MDA的含量均显著降低(P<0.01)，且呈现出一定的剂量效应关系. 结论 单独使用Vit.C的抗氧化作用，有时还会加重损伤;单独使用Vit.E具有一定的SOD活性保护和MDA清除作用，存在剂量效应关系，但对外周血细胞和免疫细胞则无明显的保护作用.

    Effect of vitamin C and E on SOD，MDA in rabbits injured by irradiation

    LIANG Xin，HAI Chun-Xu，QING Xu-Jun，ZHANG Xiao-Di，ZHAO Kang-Tao，LIU Rui，CHEN Hong-Li，SHEN Xue-Feng

    Department of Toxicology，Faculty of Preventive Medicine，Fourth Military Medical University，Xi'an710033，China

    Keywords:vitamin C;vitamin E;radiation injury;antioxida-tion;SOD;MDA

    Abstract:AIM To study the dose-effects relationship be-tween Vit.C，Vit.E，and lipid peroxidation through the mod-el of rabbit injured by radiation.METHODS Chinese rab-bits were chosen as the experimental animals and were ran-domly divided into twelve groups which each contained five.They were negative control group ......