关键词: 谷氨酸;兴奋毒性;基因，fos;视网膜;小鼠

    摘 要:目的 观察谷氨酸对小鼠视网膜神经细胞c-fos表达的变化，探讨谷氨酸的神经毒性作用. 方法 昆明系小鼠妊娠晚期经口一次性大剂量谷氨酸钠ig(4.0g·kg-1 )，仔鼠于出生后10，20和30d取眼球，多聚甲醛固定，恒冷切片，应用免疫组织化学方法对视网膜神经细胞c-fos的表达进行了观测. 结果 10，20和30日龄谷氨酸组小鼠视网膜神经细胞c-fos表达阳性神经元分别为1474，1250及859cells·mm-2 ，比正常小鼠c-fos的表达明显增高(1474vs744cells·mm-2 ，P<0.05;1250vs538cells·mm-2 ，P<0.05;859vs404cells·mm-2 ，P<0.05). 结论 外源性谷氨酸作用于成长发育中仔鼠的视网膜神经细胞，并引起细胞内c-fos过度表达，可能诱导视网膜神经细胞凋亡.

    Effect of monosodium glutamate on the expression of c┐fos in the retinal neurons of fetal mice

    XU Lei ZHAO Yan HUANG Ling XUE Rong-Liang WANG Hui-Sheng

    1 Department of Neurobiology Research，Medical School of Xi'an Jiaotong University，Xi'an710061，China，

    2 Department of Ophthalmology，the4th Hospital of Xi'an，Xi'an710004，China，

    3 Department of Anesthesiology，2nd Hospital of Xi'an Jiaotong University，Xi'an710004，China

    Keywords:glutamates;excitotoxicity;genes，fos;retina;mice

    Abstract:AIM To observe the toxic effect of monosodium glutamate on the expression of c-fos in the retinal neurons in

    mice.METHODS Kunming mice were given4.0g·kg-1 monosodium glutamate via oral administration at the late stage of pregnancy.The eyeballs of filial mice were removed on the postnatal days of10 ......