大肠癌组织RAB5A和CD44v9表达的意义
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李志高, 李晓冬, 董新舒, 庞洪刚, 杜 杰
RAB5A;CD44v9;结直肠肿瘤;分化;转移,李志高,李晓冬,董新舒,庞洪刚,杜杰,李志高,通讯作者:,SignificanceofRAB5Aan
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李志高, 李晓冬, 董新舒, 庞洪刚, 杜杰, 哈尔滨医科大学附属肿瘤医院腹外科 黑龙江省哈尔滨市 150040
李志高, 2003年哈尔滨医科大学医学博士, 副教授、硕士研究生导师, 一直从事乳腺肿瘤, 消化道肿瘤的临床治疗和防治工作.
黑龙江省自然科学基金资助项目, No.9748
通讯作者: 李志高, 150040, 哈尔滨市南岗区哈平路150号, 哈尔滨医科大学附属肿瘤医院腹外科. doctorxht@126.com
传真: 0451-86298317
收稿日期: 2005-12-17 接受日期: 2006-02-18
Significance of RAB5A and CD44v9 expression in colorectal cancer tissues
Zhi-Gao Li, Xiao-Dong Li, Xin-Shu Dong, Hong-Gang Pang, Jie Du
Zhi-Gao Li, Xiao-Dong Li, Xin-Shu Dong, Hong-Gang Pang, Jie Du, Department of Abdomen Surgery, the Affiliated Tumor Hospital of Harbin Medical University, Harbin 150040, Heilongjiang Province, China
Supported by the Natural Science Foundation of Heilongjiang Province, No.9748
Correspondence to: Zhi-Gao Li, Department of Abdomen Surgery, the Affiliated Tumor Hospital of Harbin Medical University, Harbin 150040, Heilongjiang Province, China. doctorxht@126.com
Received: 2005-12-17 Accepted: 2006-02-18
Abstract
AIM: To investigate the expression of RAB5A and CD44v9 and their significances in colorectal cancer.
METHODS: SP immunohistochemical method was adopted to detect the expression of RAB5A and CD44v9 proteins in the pathological paraffin sections from colorectal cancer (n = 43). RNA samples of 60 fresh tissues excised from patients with colorectal cancer were extracted and reversely transcribed into cDNAs, and then real-time polymerase chain reaction (RT-PCR) was used to detect the expression of RAB5A and CD44v9 genes. The correlations of RAB5A and CD44v9 expression with the differentiation and metastasis of colorectal cancer were analyzed.
RESULTS: RAB5A protein was positively expressed in cytoplasm cell membrane with a rate of 100%, while CD44v9 protein was positively expressed only in cytoplasm with a rate of 86.7%. RT-PCR results showed that Tm of each sample was coincided with that of positive control by melting curve analysis ......
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